ADOPTION OF THE « CYTOKINESIS-BLOCK MICRONUCLEUS TEST OF LIMPHOCITES » (CBMN) BIOLOGICAL DOSIMETRY METHOD

The paper provides procedure of «Micronuclear Test with Cytogenesis-Block in Lymphocytes of Peripheral Blood of Humans» cytokinesis-block micronucleus test method adoption in the Institute of Radiation Safety and Ecology of the NNC RK. Micronuclear test with blockage of cytogenesis was tested using automated cytogenetic platform based on digital fluorescent microscope Carl Zeiss AxioImager Z2, Metafer 4/MSearch, automated system for search and analysis of metaphases (MetaSystems, Germany). It was found, that base micronuclei frequency of the researched groups ranges between 2.12 and 18.1 per 1000 of binuclear cells, that corresponds to standard background values in the world (according to the bookish data).

1. Разработка и внедрение методов контроля доз персонала предприятий ядерно–топливного цикла и населения: отчет О НИР (промежуточ.) / Институт радиационной безопасности и экологии НЯЦ РК (ИРБЭ НЯЦ РК); рук. С. Н. Лукашенко; исполн.: А. Н. Шатров, Ж. А. Байгазинов. – Курчатов: ИРБЭ НЯЦ РК, 2015. – 74с. – № ГР 0115РК02082. – Инв. № 0215РК03396.

2. Beinke C., Port М., Riecke А. et al Adaption of the Cytokinesis-block micronucleus cytome assay for improved triage biodosimetry // Radiation Research. – 2016. – № 185. – P. 461–472.

3. Bhatia A., Kumar Y. Cancer cell micronucleus: an update on clinical and diagnostic applications // APMIS. – 2013. – № 121 (7). – Р. 569–581.

4. Occupational exposure to pesticides in tobacco fields: the integrated evaluation of nutritional intake and susceptibility on genomic and epigenetic instability // Oxidative Medicine and Cellular Longevity. – 2018. – ISBN № 7017423.

5. Jovicic D., Pajic J., Radivojevic L. et al. Micronucleus frequencies in peripheral blood lymphocytes in a Serbian human population exposed to pesticides // Pestic. Phytomed. – 2015. – № 30 (1). – Р. 51–60.

6. Theodore P. Rasmussen. Stem Cells in Birth Defects Research and Developmental Toxicology // John Wiley & Sons. – 2018. – ISBN № 1119283221.

7. Bonassi S., Fenech М., Lando С. et al. HUman MicroNucleus Project: international database comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes: I. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei // Environmental and Molecular Mutagenesis. – 2001. – № 45. – Р. 31–45.

8. Depuydt J., Baeyens А., Barnard S. Reneb intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay) / J. Depuydt, А. Baeyens, S. Barnard [et al] // Int. Journal of radiation biology. – 2016. – № 93. – P. 36-47.

9. H. Thierens., A. Vral., M. Barbe., B. Aousalah., L. De Ridder. A cytogenetic study of nuclear power plant workers using the micronucleus-centromere assay// Mutation Research. – 1999. – № 445. – P. 105–111.

10. Stefano Bonassi., Monica Neri., Cecilia Lando., Marcello Ceppi. et al. Effect of smoking habit on the frequency of micronuclei in human lymphocytes: results from the Human MicroNucleus project // Mutation Research. – 2003. – № 543. – P. 155–166.