ASSESSMENT OF THE DEGREE OF DNA DAMAGE AND REPAIR RESIDENTS DOLON WITH COMET-TEST
https://doi.org/10.52676/1729-7885-2019-3-134-138
Abstract
The results of a study of the degree of DNA damage in peripheral blood lymphocytes in residents of Dolon (Semipalatinsk region, East Kazakhstan region) are presented. The spontaneous level was analyzed after in vitro exposure to γ-radiation at a dose of 1 Gy and the assessment of the efficiency of repair 2.5 hours after irradiation. A cytogenetic study of the spontaneous frequency of chromosomal aberrations and radiosensitivity with additional radiation exposure was also conducted. A significant increase in the degree of DNA damage and cytogenetic disorders in the inhabitants of the Dolon pod was shown compared with the control in the study of the spontaneous level. The repair efficiency, determined usinga Comet-test and radiosensitivity based on cytogenetic analysis with additional irradiation of the cells of the patients, varied significantly within different individuals. These parameters in specific individuals may be of great importance for individual retrospective dose assessment during acute or chronic exposure or when living in radiocontaminated regions. A comparative analysis of the data obtained using the methods used revealed a significant positive correlation between the spontaneous frequency of chromosomal abnormalities and the efficiency of repair.
About the Authors
O. G. Cherednichenko
Institute of General Genetics and Cytology, CN, MES RK
Kazakhstan
Almaty
Kazakhstan
Almaty
A. L. Pilyugina
Institute of General Genetics and Cytology, CN, MES RK
Kazakhstan
Almaty
Kazakhstan
Almaty
References
1. Газиев А.И. Низкая эффективность репарации критических повреждений ДНК, вызываемых малыми дозами радиации//Радиационная биология. Радиоэкология. – 2011. – Т. 51, № 5. – С. 512–529.
2. Сирота Н.П., Кузнецова Е.А. Применение метода “Комета тест” в радиобиологических исследованиях //Радиационная биология. Радиоэкология. – 2010 – Т. 50, № 3. – С. 329–339. 3. Рябченко Н.Н., Антощина М.М., Насонова В.А. и др. Аберрации хромосом в лимфоцитах человека при различной продолжительности культивирования после облучения // Радиационная биология. Радиоэкология. – 2004. – Т. 44, № 2. – С. 146–150.
3. Cerqueira E.M., Gomes-Filho I.S., Trindade S., Lopes M.A., Passos J.S., Machado-Santelli G.M. Genetic damage in exfoliated cells from oral mucosa of individuals exposed to X-rays during panoramic dental radiographies // Mutat. Res. – 2004. – Vol. 562, № 1–2. – P. 111–117.
4. Godderis L., Aka P., Mateuca R., Kirsch-Volders M., Lison D., Veulemans H. Dose-dependent influence of genetic polymorphisms on DNA damage induced by styrene oxide, ethylene oxide and gamma-radiation // Toxicology. – 2006. – Vol. 219, № 1–3. – Р. 220–229.
5. Struwe M., Greulich K.O., Suter W., Plappert-Helbig U. The photo comet assay – A fast screening assay for the determination of photo genotoxicity in vitro // Mutat. Res. – 2007. - Vol. 632, № (1–2). – Р. 44–57.
6. Оценка генотоксических свойств методом ДНК-комет in vitro. Методические рекомендации. – М.: Федеральный центр гигиены и эпидемиологии Роспотребнадзора, 2010. – 15 с.
7. Struwe M., Greulich K.O., Suter W., Plappert-Helbig U. The photo comet assay-A fast screening assay for the determination of photo genotoxicity in vitro // Mutat. Res. – 2007. - Vol. 632, № (1–2). – Р. 44–57.
8. Moorhead P.S., Nowell P.C., Mellman W.J., Battips D.M., Hungerford D.A. Chromosome preparations of leucocytes cultured from human peripheral blood // Experimental Cell Research. 1960. – Vol. 20. – Р. 613–616.
9. Плохинский Н.A. Aлгоритмы в биометрии. – М.: МГУ, 1967. – 82 с.
10. Губицкая Е.Г., Чередниченко О.Г., Байгушикова Г.М., Ахматуллина Н.Б. Цитогенетический статус жителей Алматинской области // Вестник Каз. НУ им. аль-Фараби. Серия биологическая. – 2007. –№ 2. – С. 86–90.
11. Губицкая Е.Г., Ахматуллина Н.Б., Всеволодов Э.Б., Вишневская С.С., Шарипов И.К. Частота аберраций хромосом у жителей районов, прилегающих к Семипалатинскому испытательному ядерному полигону //Генетика. – 1999. – № 6. – С. 842–846.
12. Zakeri F., Hirobe T.A. Cytogenetic approach to the effects of low levels of ionizing radiations on occupationally exposed individuals // European Journal of Radiology. – 2010. – Vol. 73, I. 1. – P. 191–195.
Review
For citations:
Cherednichenko O.G., Pilyugina A.L. ASSESSMENT OF THE DEGREE OF DNA DAMAGE AND REPAIR RESIDENTS DOLON WITH COMET-TEST. NNC RK Bulletin. 2019;(3):134-138. (In Russ.) https://doi.org/10.52676/1729-7885-2019-3-134-138
Views:
286
Email this article 